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. 2015 Aug 22;106(9):1182–1187. doi: 10.1111/cas.12734

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© 2015 The Authors. Cancer Science published by Wiley Publishing Asia Pty Ltd on behalf of Japanese Cancer Association.

This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Induction of pluripotent cancer cells from PANC-1 cells. (a) Schematic representation of the reprogramming protocol. (b) RT-PCR analysis of transgenes for reprogramming. (c) Representative image of induced pluripotent stem (iPS)-like colonies. Phase, phase contrast. Bar = 500 μm. (d) Representative image of alkaline phosphatase (ALP)-positive colonies. Bar = 500 μm. (e) Immunofluorescence staining of ES cell markers in reprogrammed colonies. Right panels, Hoechst33342. (f) qRT-PCR of differentiation markers. n = 3. Data are represented as the mean ± SD and were analyzed by two-tailed unpaired t-tests. (*P<0.05, **P<0.01, P-iC vs. NC), P-iC, Post-iPC; NC, Negative control.Bar = 500 μm. ALP, alkaline phosphatase; Ctrl, control; Day 3, post-transduced PANC-1 cells on day 3; iPS, induced pluripotent stem; SeV-cMYC, Sendai virus carrying cMYC; SeV-KOS, Sendai virus carrying polycistronic transgenes KLF4-OCT3/4-SOX2.