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. 2015 Aug 6;106(9):1188–1195. doi: 10.1111/cas.12733

Figure 3.

Figure 3

Overexpression of miR-367 induces generation of medulloblastoma neurospheres. (a) Amount of neurospheres generated from medulloblastoma cells transfected with either miR-367 mimic or non-specific control, after 4 days of culture in neural stem cell media. (b) Representative images of CHLA-01-Med, USP-13-Med, D283-Med and Daoy neurospheres. (c) Proportion of CD133+ cells in medulloblastoma neurospheres, assessed by flow cytometry. CHLA-01-Med, USP-13-Med, D283Med and Daoy neurospheres were enriched in cells expressing CD133, with 91.7%, 90.3%, 87.4% and 48.2% of CD133+ cells, respectively. Scale bar: 200 μm. Significance level: *P < 0.05, **P < 0.01, ****P < 0.0001.