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(a) Quantitative PCR test showed a highly consistent expression of β-globin copies in nasopharyngeal (NP) brushing samples between nasopharyngeal carcinoma (NPC) patients and controls, indicating that NP brush sampling was a reliable method. (b) A significant higher Epstein–Barr virus (EBV) DNA load was observed in NPC patients (P < 0.0001), compared to controls. No significant difference was observed between non-NPC control and high-risk control groups.
