FIGURE 2.

Influence of rWTX and its mutants on [³H]NMS binding to mAChRs. Results are given as means ± S.E. of 3–7 values expressed in percent of control binding without toxin obtained in the same experiment. Dashed lines indicate 95% confidence interval of control binding that was in pmol/mg protein: M1, 1.81 ± 0.06, n = 12; M2, 0.28 ± 0.01, n = 9; M3, 1.46 ± 0.05, n = 9; M4, 0.49 ± 0.01, n = 9; M5, 0.32 ± 0.01, n = 9. A single concentration (10 μm) of the toxins was tested. A, comparison of effects of wild-type WTX and rWTX on [³H]NMS binding at mAChRs. No effects of wild-type WTX on [³H]NMS binding with M2, M4, and M5 receptors were previously reported (15). Data indicated as follows: #, p < 0.05; ##, p < 0.01, and ###, p < 0.001 are significantly different from each other. B–D, all mutants were compared with rWTX and control by analysis of variance followed by Dunnett's multiple comparisons test. Data indicated as * and ° (p < 0.05), ** and °° (p < 0.01), and *** and °°° (p < 0.001) are significantly different from rWTX and control, respectively.