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Root-mean-square fluctuation (RMSF) of rWTX(P33A) (A) and mAChRs (B). Free and bound rWTX(P33A) and M1-mAChR are shown with black and green lines, respectively. Free M3-mAChR is shown with purple line. For rWTX(P33A) and mAChRs, location of loops I–III and TM α-helices, respectively, is shown. Intracellular loop 3 of the receptor located between TM helices 5 and 6 is absent from the model. Amino acid residue numbering is provided for rWTX(P33A) and M1 receptor.
