FIGURE 2.

Luteolin suppresses HNF4α activity. HepG2 cells (A, B, and D) and HEK293 cells (C) were transfected with 200 ng of the indicated reporter constructs and 200 ng of pCMV-β-Gal (A–D) and 200 ng of pFLAG-HNF4α (C) or 200 ng of pGal4 DBD-HNF4α LBD (D). The cells were cultured with medium A for 24 h and then treated with the indicated compound for 12 h. Luciferase assays were performed as described under “Experimental Procedures.” All data are expressed as means ± S.E. (error bars) (n = 3). **, p < 0.01.