FIGURE 6.

Luteolin glucosides do not suppress apoB secretion from HepG2 cells. A, structure of luteolin, luteolin 7-glucoside, and isoorientin. B, HepG2 cells were transfected with plasmids as described in Fig. 1A and then treated with the indicated compounds for 12 h. Luciferase assays were performed as described under “Experimental Procedures.” C, HepG2 cells were cultured with the indicated compounds for 12 h, and total RNA was isolated. Real-time PCR analysis was performed, and relative mRNA levels were obtained after normalization of 36B4 mRNA. The mRNA levels without luteolin addition are represented as 1. All data are expressed as means ± S.E. (error bars) (n = 3). *, p < 0.05; **, p < 0.01. D, HepG2 cells were cultured with the indicated compounds for 12 h, and media were isolated and subjected to SDS-PAGE and immunoblotting (IB) with anti-apoB antibodies. The same results were obtained in three separate experiments.