FIGURE 9.

Dietary luteolin suppresses diet-induced obesity and improves glucose tolerance. A, change in the body weight of male mice fed HFD or HFD with 0.6 and 1.5% luteolin for 57 days (n = 8). The arrows represent the points when blood glucose measurement, CT, and OGTT were performed. B, at day 44 after luteolin supplementation, CT was performed as described under “Experimental Procedures.” C, at day 57 after luteolin supplementation, the mice were fasted for 4 h and sacrificed. Proteins in serum were analyzed by immunoblotting (IB) with anti-apoB antibodies (n = 8). The typical samples (n = 3) were represented. D, the signals (n = 8) detected on the membrane in C were quantified. The signals of the HFD group are represented as 1. E, at day 49 after luteolin supplementation, OGTT was performed as described under “Experimental Procedures.” F, the average of area under the curve (AUC). All data are expressed as means ± S.E. (error bars) (n = 8). Means without a common letter differ (p < 0.05).