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Kinase and ATPase activities of wild-type and mutant A-CAT. A, kinase and ATPase activity of wild-type (WT) A-CAT and the indicated mutants were assayed as described under “Experimental Procedures.” B, ATPase and kinase activities for the WT A-CAT and the R592L and K722N mutants were assayed at varying ATP concentrations. The kinetic constants obtained by fitting the data to a hyperbolic curve are listed in Table 3.
