TABLE 3.

Hydrolytic and kinase activities for wild-type and mutant A-CAT

A-CAT	Nucleotide	Hydrolysis activity		Kinase activity
		kcat	Km, ATP	kcat	Km, ATP
		min−1	μm	min−1	μm
WT	ATP	1.92 ± 0.09	35 ± 4	5.8 ± 0.4	23 ± 5
WT	ADP	0.61 ± 0.05	71 ± 23
WT	AMP	0.32 ± 0.04	270 ± 90
R592L	ATP	0.54 ± 0.03	48 ± 7	1.8 ± 0.2	58 ± 18
K722N	ATP	0.32 ± 0.04	70 ± 10	1.0 ± 0.2	63 ± 27