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Purification of PPi-PEPCK from E. histolytica
1 unit represents the amount of enzyme required to oxidize 1 μmol of NADH/min.
| Activity | Specific activity | Purification | Yield | |
|---|---|---|---|---|
| units | units/mg | -fold | % | |
| 100,000 ×g supernatant | 75.4 | 0.165 | 1.0 | 100 |
| Butyl-Toyopearl | 32.1 | 4.86 | 29.4 | 42.6 |
| MonoQ | 8.06 | 34.0 | 206 | 10.7 |
