TABLE 3.
Relative quantification of EhPEPCK isotypes
| Peptide | Peak area of monoisotopic ion |
Normalized relative ratio against common peptides |
||||
|---|---|---|---|---|---|---|
| EhPEPCK1-HA IP | EhPEPCK2-HA IP | Purification from cells | EhPEPCK1-HA IP | EhPEPCK2-HA IP | Purification from cells | |
| Unique to EhPEPCK1 | ||||||
| GTDYPILNIQELEALADLK | 4.54E+07 | 3.96E+06 | 4.61E+06 | |||
| SDAIDVVAPLVDIIAEGDQESTAPIDAR | 4.09E+07 | 6.45E+06 | 2.17E+06 | |||
| NISDAIFEGK | 2.41E+09 | 3.91E+08 | 6.71E+08 | |||
| Sum | 2.50E+09 | 4.01E+08 | 6.78E+08 | 3.0E-01 | 5.5E-02 | 1.1E-01 |
| Specific to EhPEPCK2 and -3 | ||||||
| ITTAFANHFLR | 9.22E+07 | 6.07E+08 | 1.68E+08 | |||
| KLESLANLK | 3.81E+08 | 1.76E+09 | 8.54E+08 | |||
| LAGPLLEEVEESEVNHTTAPIDAR | 2.62E+08 | 1.03E+09 | 2.54E+08 | |||
| Sum | 7.35E+08 | 3.40E+09 | 1.28E+09 | 8.8E-02 | 4.6E-01 | 2.1E-01 |
| Unique to EhPEPCK2 | ||||||
| AVQEIFDHDFSKR | 5.79E+06 | 2.38E+07 | 2.05E+07 | 6.9E-04 | 3.3E-03 | 3.3E-03 |
| Unique to EhPEPCK3 | ||||||
| GANLSSQYLR | 2.14E+08 | 9.04E+06 | 3.37E+08 | 2.6E-02 | 1.2E-03 | 5.5E-02 |
| Common to all | ||||||
| YLIEHGYLEPCPDVTYNGK | 2.15E+08 | 1.87E+08 | 3.52E+08 | |||
| LFQRPDDAVFR | 4.41E+09 | 4.39E+09 | 2.91E+09 | |||
| DFFPAAK | 3.71E+09 | 2.74E+09 | 2.92E+09 | |||
| Sum | 8.34E+09 | 7.32E+09 | 6.18E+09 | 1.0 | 1.0 | 1.0 |