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Activation of atypical neddylation accumulates a proteasome surrogate substrate GFPu. A and B, HEK293 cells stably expressing GFPu and RFP were transfected with the SF-NEDD8 or SF-NEDD8ΔGG expression vectors for 48 h. Conj, conjugate. C and D, NRVMs were infected with Ad-GFPu and Ad-RFP before infection with Ad-SF-NEDD8 for another 48 h. Representative immunoblots of the indicated proteins (A and C) and quantitative analyses (B and D) are shown. **, p < 0.01; ***, p < 0.001.
