FIGURE 6.

NUB1L reduces misfolded protein CryAB^R120G in cardiomyocytes. NRVMs were infected with Ad expressing HA-tagged CryAB^R120G (Ad-HA-CryAB^R120G) for 24 h and subsequently infected with Ad-NUB1L (20 MOI) or transfected with siRNA against NUB1L (siNUB1L) for another 72 h. A—D, the Triton X-100 (1%) soluble and insoluble fractions of cell extracts were subjected to Western blot analysis (A and C) and protein dot blot to quantify the abundance of CryAB^R120G (B and D). Arrows point to the modified forms of CryAB^R120G. Exp, exposure.