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. 2015 Aug 10;290(39):23850–23862. doi: 10.1074/jbc.M115.664375

FIGURE 7.

FIGURE 7.

NUB1L promotes the proteasomal degradation of CryABR120G. NRVMs were infected with Ad-HA-CryABR120G or WT CryAB for 24 h and subsequently infected with Ad-NUB1L (20 MOI). A and B, Western blots of total cell extracts (A) and quantification analysis (B). NUB1L overexpression reduced CryABR120G but not WT-CryAB protein levels. Endo, endogenous; N.S., not significant. C, quantitative PCR analysis of CryABR120G transcripts. D and E, proteasome inhibition attenuates the NUB1L-induced reduction of CryABR120G. Representative Western blots (D) of total cell extracts and densitometric analysis (E) are shown. BZM was added 24 h before cell harvesting. F and G, CHX chase assay. Representative Western blots of total cell extracts (F) and pooled densitometry data (G) of CryABR120G are shown. The changes of the native form and modified forms (arrows) of CryABR120G are shown in the short and long exposure (expos) blots, respectively. The abundance of NUB1L is denoted under the blot. A.U., arbitrary unit.