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. 2015 Aug 3;290(39):23680–23691. doi: 10.1074/jbc.M115.676734

FIGURE 2.

FIGURE 2.

Offspring exchange breeding scheme and analysis. A, breeding scheme used to generate WT pups receiving WT milk, WT pups receiving KO milk, KO pups receiving WT milk, and KO pups receiving KO milk. Pups were given to foster mothers within 1 day of birth. The breeding scheme was carried out using both 375KO and 200cKO mouse models. B and C, miR-375 copy number per nanogram of RNA in D14 gastric milk (B) and D3 gastric milk (C) from pups of the miR-375 pup exchange (n = 4). D, miR-200c copy number per nanogram of RNA in D14 gastric milk from pups of the miR-200c pup exchange (n = 4). E, Northern blot analysis of miR-375 expression in D14 milk (14 μg of RNA pooled from three to four pups), with mammary tissue (14 μg of RNA) used as the positive control. miR-200c expression was measured as RNA loading control. F, density quantification of miR-375 Northern blot analysis normalized to the mammary gland. Rel, relative. B–D, results are represented as mean ± S.E. *, p ≤ 0.05; **, p < 0.01; ***, p < 0.001; ns, p > 0.05.