FIGURE 3.

Kinetic analysis of the thiosulfate:cyanide sulfurtransferase reaction catalyzed by rhodanese and its polymorphic variants. A, C, and E, dependence of the reaction velocity on cyanide concentration in the presence of 100 mm thiosulfate (A, wild type; C, E102D; and E, P285A rhodanese). B, D, and F, dependence of the reaction velocity on thiosulfate concentration in the presence of saturating (100 mm) cyanide (B, wild type; D, E102D; and F, P285A rhodanese). The reaction conditions are described under “Experimental Procedures,” and the data are representative of three independent experiments.