FIGURE 5.

Kinetic analysis of the thiosulfate:thiol sulfurtransferase reactions catalyzed by the rhodanese polymorphic variants. A–F, dependence of the reaction velocity of sulfur transfer from 3 mm thiosulfate on varying concentrations of GSH (A and D), cysteine (B and E), or homocysteine (C and F) for E102D (A, C, and E) and for P285A (B, D, and F) rhodanese. The reaction conditions are described under “Experimental Procedures,” and the data are representative of three independent experiments.