FIGURE 6.

Dependence of the thiosulfate:thiol sulfurtransferase reactions catalyzed by the rhodanese polymorphic variants on thiosulfate concentration. A–F, the kinetics of sulfur transfer from varying concentrations of thiosulfate in the presence of 50 mm GSH (A and B), 50 mm l-cysteine (C and D), or 50 mm l-homocysteine (E and F) for E102D (A, C, and E) and P285A (B, D, and F) rhodanese. The reaction conditions are described under “Experimental Procedures.” The data are representative of three independent experiments.