Working model to explain the structure-function relationship of OsGAP1.
A, the structure of OsGAP1 is shown in a surface representation. Mutations in Clusters 1 (green) and 3 (blue) abolished the interaction with OsYchF1, whereas mutations in Clusters 2 (red), 3, 4 (cyan), and 5 (yellow) abolished/reduced the phospholipid binding of OsGAP1. B, the interaction of OsGAP1 and OsYchF1 on the plasma membrane is involved in plant defense responses. C, the interaction of OsGAP1 and OsYchF1 in the cytosol is involved in salt stress responses.