FIGURE 5.
Temperature dependence of phase relaxation rate of [2Fe-2S] cluster in native and mutated cytochrome bc1. A, G167P (red closed squares) and WT (orange closed squares). B, 2Ala (blue open triangles), G167P/2Ala (blue closed triangles), 1Ala (dark green open diamonds), G167P/1Ala (green closed diamonds), G167P (red closed squares), and WT (orange closed squares) (last two sets of data are replotted from A). Control sample (black closed circles) is WT in which there are no dipolar interactions between heme bL and [2Fe-2S] cluster. All samples in A and B were reduced with ascorbate except for the control of WT that was reduced with dithionite.