Fig. 6.

High-level hMsrA in the liver attenuates hepatic lipid content and fecal cholesterol level in apoE^−/− mice. Lv-GFP and Lv-MsrA-GFP mice were fed a Western diet for 12 weeks. Livers were harvested at the time of euthanasia and either used to extract total lipids or fixed for histochemical analysis. A, B: Histochemical analysis of fresh liver tissue was performed by staining with HE or Oil Red O (5 µm serial sections). C: The amounts of TC, FC, CE, and TG were determined using iospropanol as described in Methods and expressed as milligrams per gram of liver protein (mean ± SD, n = 6). D: Total fecal cholesterol was determined as described in Methods and expressed as milligram per gram of dry feces. * P < 0.05 and ** P < 0.01.