Fig. 1.

Impact of the FAAH inhibitors URB597 and AA-5HT on the migration of MSCs. A, B: Time-course of migration of MSCs treated with vehicle, 10 μM URB597 (A), or 10 μM AA-5HT (B) in Boyden chamber assays. C, D: Concentration-dependent impact of URB597 and AA-5HT on migration of MSCs (Boyden chamber assays). MSCs were incubated with vehicle, URB597 (C), or AA-5HT (D) at the indicated concentrations for 6 h. E: Phase contrast images of migration of MCSs into scratched areas following a 24 h (URB597) or 12 h (AA-5HT) incubation with the respective FAAH inhibitor or its vehicle. The indicated images depict one representative experiment. t0, the untreated cells immediately after scratching the cells. Data represent mean ± SEM compared with vehicle controls (100%) of n = 3–4 experiments with cells from one donor (A, B), n = 6–7 experiments with cells from two donors (C, D; vehicle, 1 μM and 10 μM), and n = 3–4 experiments with cells from one donor (C, D; vehicle, 0.01 μM and 0.1 μM), respectively. With respect to different vehicle controls in (C) and (D) that were considered for statistical evaluation of the respective experiment, vehicle bars in (C) and (D) do not contain SEM. *P < 0.05; Student’s t-test (A, B) and one-way ANOVA plus post hoc Dunnett test (C, D).