Fig. 5.
Competition of binding of [14C]C18:1-CoA. Binding assays were performed as described in legend of Fig. 1, and 0.1% Triton X-100 was added to the buffer when fatty acids were used. A: Binding of 25 µM [14C]C18:1-CoA to ACBD6 was measured in the presence of increasing concentrations of C18:1-CoA (red circles), C20:4-CoA (blue triangles), and C18:1-OH (green diamonds). B: Binding of 25 µM [14C]C18:1-CoA to ACBD6 was measured in the presence of 100 µM of the indicated fatty acids. Binding activity values are reported as percentage of the difference of the amount of bound [14C]C18:1-CoA to ACBD6 in presence of the unlabeled species relative to the amount obtained in their absence. Error bars represent the standard deviations of at least three measurements.