TABLE 2.

Calibration data

	Calibration Data (Plasma Matrix)				LOD (pmol/μl)	LOQ (pmol/μl)	Calibration Data (Liver Matrix)				LOD (pmol/mg w.w.)	LOQ (pmol/mg w.w.)
	Calibration Range (pmol/μl)	IS (pmol/μl)	Slope	R2			Calibration Range (pmol/mg w.w.)	IS Added (pmol/mg w.w.)	Slope	Correlation Coefficient (R2)
C0	1.5–500	7.70	0.28	0.999	0.089	0.266	1.5–1,000	180.55	0.0088	0.990	0.275	0.824
C2	0.375–125	3.21	0.34	0.999	0.038	0.113	0.375–250	75.21	0.0099	0.999	0.074	0.222
C3	0.075–25	0.84	1.37	0.999	0.008	0.024	0.075–50	19.78	0.0389	0.999	0.003	0.008
C4	0.075–25	0.42	3.40	0.999	0.008	0.023	0.075–50	9.89	0.098	0.999	0.008	0.011
2-M-C3	0.075–25	0.42	3.49	0.999	0.007	0.021	0.075–50	9.89	0.101	0.999	0.004	0.012
C5	0.075–25	0.38	5.67	0.999	0.006	0.018	0.075–50	8.97	0.159	0.999	0.003	0.009
3-M-C4	0.075–25	0.38	5.95	0.999	0.007	0.021	0.075–50	8.97	0.158	0.999	0.003	0.009
C6	0.075–25	0.45	3.18	0.999	0.013	0.038	0.075–50	10.58	0.098	0.999	0.008	0.024
C8	0.075–25	0.41	2.27	0.997	0.006	0.020	0.075–50	9.66	0.071	0.999	0.002	0.006
C10	0.075–25	0.45	2.43	0.993	0.009	0.028	0.075–50	10.58	0.083	0.999	0.005	0.014
C12	0.15–50	0.42	2.45	0.992	0.009	0.027	0.15–100	9.89	0.093	0.999	0.004	0.012
C14	0.15–50	0.40	2.56	0.996	0.009	0.028	0.15–100	9.43	0.104	0.999	0.003	0.009
C16	0.15–50	0.80	1.80	0.998	0.002	0.007	0.15–100	18.86	0.064	0.999	0.002	0.005
C18	0.15–50	0.73	1.31	0.994	0.021	0.065	0.15–100	17.02	0.049	0.999	0.021	0.063

Calibration lines were generated by plotting the area ratios of analyte/IS against the spiked concentrations. Concentrations in liver are given per milligram of liver wet weight. w.w., wet weight.