TABLE 3.
Liver Spiked (pmol/mg w.w.) | Accuracy (%) | Plasma Spiked (pmol/μl) | Accuracy (%) | |
C0 | 30 | 261.8 | 20 | 119.7 |
150 | 87.4 | 150 | 95.5 | |
500 | 89 | 300 | 97.8 | |
C2 | 7.5 | 106.7 | 5 | 116.1 |
37.5 | 102.7 | 37.5 | 97.7 | |
125 | 97.1 | 75 | 97.9 | |
C3:0 | 1.5 | 104.2 | 1 | 116.3 |
7.5 | 98.1 | 7.5 | 94.2 | |
25 | 98.7 | 15 | 97.8 | |
C4:0 | 1.5 | 96.2 | 1 | 115.5 |
7.5 | 101 | 7.5 | 92.2 | |
25 | 101 | 15 | 93.3 | |
2-M-C3:0 | 1.5 | 82.9 | 1 | 120.0 |
7.5 | 97.2 | 7.5 | 93.3 | |
25 | 96.4 | 15 | 93.9 | |
3-M-C4:0 | 1.5 | 84.3 | 1 | 113.9 |
7.5 | 94.3 | 7.5 | 89.5 | |
25 | 94.2 | 15 | 96.0 | |
C5:0 | 1.5 | 84.1 | 1 | 108.3 |
7.5 | 95.8 | 7.5 | 91.8 | |
25 | 94.1 | 15 | 97.6 | |
C6:0 | 1.5 | 90.8 | 1 | 115.4 |
7.5 | 100.8 | 7.5 | 102.6 | |
25 | 102 | 15 | 100.6 | |
C8:0 | 1.5 | 90.3 | 1 | 110.1 |
7.5 | 105 | 7.5 | 104.7 | |
25 | 107.9 | 15 | 100.9 | |
C10:0 | 1.5 | 86.3 | 1 | 109.8 |
7.5 | 99.3 | 7.5 | 103.6 | |
25 | 98.1 | 15 | 106.6 | |
C12:0 | 3 | 87.2 | 2 | 108.7 |
15 | 95.8 | 15 | 107.5 | |
50 | 96.5 | 30 | 105.7 | |
C14:0 | 3 | 89.3 | 2 | 119.1 |
15 | 91.7 | 15 | 109.2 | |
50 | 90 | 30 | 105.8 | |
C16:0 | 3 | 81.7 | 2 | 112.3 |
15 | 90.3 | 15 | 102.4 | |
50 | 92.4 | 30 | 102.1 | |
C18:0 | 3 | 82.5 | 2 | 107.8 |
15 | 99.6 | 15 | 106.0 | |
50 | 98.5 | 30 | 104.0 |
The displayed accuracy is the average of the assayed concentration (corrected by endogenous levels of plasma and liver samples) in percent of the actual spiked concentration. Concentrations in liver are given per milligram of liver wet weight. w.w., wet weight.