TABLE 2.
Validation results of CT and 7-KC by HPLC-ESI-MS/MS
| CT | 7-KC | |||
| Calibration curve, n = 5 | ||||
| Mean slope (range; SD) | 0.0080 (0.0074–0.0089; 0.0006) | 0.0044 (0.0037–0.0049; 0.0005) | ||
| Mean intercept (range; SD) | 0.39 (0.29–0.44; 0.061) | 0.10 (0.09–0.12; 0.013) | ||
| Mean coefficient of linear regression, r2 (range; SD) | 0.9908 (0.9830–0.999; 0.006) | 0.9926 (0.9817–0.9975; 0.0058) | ||
| Imprecision (% CV) | Accuracy (%) | Imprecision (% CV) | Accuracy (%) | |
| Within day, n = 10 | ||||
| 12.5 ng/ml | 5.5 | 3.8 | 5.5 | 0.3 |
| 100 ng/ml | 3.6 | 1.7 | 4.4 | 3.7 |
| 800 ng/ml | 2.4 | 8.9 | 3.9 | 4.4 |
| Between day, n = 10 | ||||
| 12.5 ng/ml | 5.8 | 1.5 | 5.8 | 4 |
| 100 ng/ml | 5.8 | 5.3 | 7.9 | 6.4 |
| 800 ng/ml | 2.9 | 0.5 | 7.7 | 5.4 |
| Recovery (%), n = 3 | ||||
| 25 ng/ml | 94.4 | 94 | ||
| 200 ng/ml | 98.2 | 107 | ||
| 400 ng/ml | 91.8 | 100.7 | ||
| LOD (ng/ml), n = 3 | 1 | 1 | ||
| LOQ (ng/ml), n = 3 | 3 | 3 | ||