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. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: Angiogenesis. 2014 Aug 20;17(4):779–804. doi: 10.1007/s10456-014-9440-7

Figure 8.

Figure 8

CAM cultivation protocols. The ex ovo protocol (a–c) requires (a) a gentle cracking of the egg using a blade and transferring of the contents into a petri dish or weighing boats (b) with plastic a plastic cover (c). The in ovo cultivation (d–f) starts with gentle rotation for 3 days. On embryo development day 3 the eggshell is opened with a sterile tweezers (d) and subsequently covered with a laboratory wrapping film (e). On the day when the experiment starts, the shell above the air pouch of the egg is extended to a diameter of approximately 3 cm (f) enabling further experimental manipulation.