Table 2.

Primers used for real-time RT-PCR

Target	Sense primer	Antisense primer
RPLP0	GGAAACTCTGCATTCTCGCTTCCT	CCAGGACTCGTTTGTACCCGTTG
GAPDH	CCTCCTGCACCACCAACTGCTT	GAGGGGCCATCCACAGTCTTCT
IGF-IEa	GACATGCCCAAGACCCAGAAGGA	CGGTGGCATGTCACTCTTCACTC
IGF-IEc	GCCCCCATCTACCAACAAGAACAC	CGGTGGCATGTCACTCTTCACTC
HGF	TGAAATATGTGCTGGGGCTGAAA	ACAAACAAGTGGGCCACCATAATCC
Cmet	AACCCGAATACTGCCCAGACCC	TGATATCCGGGACACCAGTTCAG
MCP1	GCCCTTCTGTGCCTGCTGCT	GCAGGTGACTGGGGCATTGATT
COX1	GGTTTGGCATGAAACCCTACACCT	CCTCCAACTCTGCTGCCATCT
COX2	TGGAACATGGAATTACCCAGTTTGTTG	TGTGATACTTTCTGTACTGCGGGTGG
HSP70	GTGGCTGGACGCCAACACCTT	TTACACACCTGCTCCAGCTCCTTC
HSP27	GCTGACGGTCAAGACCAAGGATG	TGAAGCACCGGGAGATGTAGCC
αB-crystallin	GTGTTGGGAGATGTGATTGAGGTG	CTGGGATCCGGTATTTCCTGTGG
Myogenin	CTGCAGTCCAGAGTGGGGCAGT	CTGTAGGGTCAGCCGTGAGCAG
Myf6	GGGCTCGTGATAACGGCTAAGGA	TGTCCACGATGGAAGAAAGGCA
MyoD	ACGAAGGCGCCTACTACAACGA	GACACCGCCGCACTCTTCCC
Myostatin	TGCTGTAACCTTCCCAGGACCA	GCTCATCACAGTCAAGACCAAAATCC
Atrogin1	TGTTACCCAAGGAAAGAGCAGTATGGA	ACGGAGCAGCTCTCTGGGTTATTG
MuRF1	TGGGGGAGCCACCTTCCTCT	ATGTTCTCAAAGCCCTGCTCTGTCT
TNFα	TTCCCCAGGGACCTCTCTCTAATC	GAGGGTTTGCTACAACATGGGCTAC
TNFRI	GGGAGGACAGCGCCCACAAG	CACGAATTCCTTCCAGCGCAAC
TNFRII	CCACTCGGAACCAGCCACAG	CCATGGCCACCAGGGGAAGA
IL-1β	TGCGTGTTGAAAGATGATAAGCCCA	CAAATCGCTTTTCCATCTTCTTCTTTG
IL-1R	GGAAGGGATGACTACGTTGGGGA	CCAGCCAGCTGAAGCCTGATGTT
IL-6	GAGGCACTGGCAGAAAACAACC	CCTCAAACTCCAAAAGACCAGTGATG
IL-6R	CCAGGAGGAGTTCGGGCAAG	GGGGTGGACACCTCGTTCTCA

Abbreviations: RT-PCR real-time reverse transcriptase-polymerase chain reaction, RPLP0 large ribosomal protein P0, GAPDH glyceraldehyde-3-phosphate dehydrogenase, IGF insulin-like growth factor, HGF hepatocyte growth factor, MCP1 monocyte chemoattractant protein = CCL2, COX cyclooxygenase, HSP heat shock protein, MuRF1 muscle ring finger protein 1, TNF tumor necrosis factor, IL interleukin, R receptor

The HGF primers are specific for the two largest splice variants including the SP domain