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. 2015 Sep 25;10(9):e0139277. doi: 10.1371/journal.pone.0139277

Fig 1. Impact of temperature on the short-term survival of Lp in Fraquil and intracellular multiplication (ICM) after exposure to different temperatures.

Fig 1

A) The JR32 strain was exposed to Fraquil at six different temperatures. Weekly CFU counts were performed to track survival. DL, detection limit. At 4°C, 10°C, 37°C and 42°C, CFU counts from 8 days were statistically different (P≤0.05) than CFU counts at 25°C. At 30°C, CFU counts from day 42 were statistically different that CFU counts at 25°C. B) A. castellanii was infected with JR32 that had been exposed to the respective temperatures tested for 7 days or 28 days at an MOI of 0.1. Daily CFU counts monitored the ICM inside amoeba and are presented as the ratio over CFU counts at day 0. JR32 from BCYE was used as the positive control and dotA - was used as the negative control. C) Cultured human macrophages (THP-1) were infected with JR32 that had been exposed to the respective temperatures tested for 7 days or 28 days at an MOI of 0.1. Daily CFU counts monitored the ICM and are presented as the ratio over CFU counts at day 0. JR32 from BCYE was used as the positive control and dotA - was used as the negative control.