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. 2015 Sep 25;10(9):e0139277. doi: 10.1371/journal.pone.0139277

Fig 2. Survival of Lp at different trace metal concentrations and subsequent intracellular multiplication (ICM).

Fig 2

A) The JR32 strain of Lp was exposed to Fraquil at four different metal concentrations: no addition of trace metals (0X), standard Fraquil (1X), double the quantity of trace metals (2X) and 10 times the quantity of trace metals (10X) than in standard Fraquil. Weekly CFU counts were performed to track survival. DL, detection limit. B) A. castellanii was infected with JR32 that had been exposed to the respective levels of trace metals tested for 30 days at an MOI of 0.1. Daily CFU counts monitored the ICM inside amoeba and are presented as the ratio over CFU counts at day 0. JR32 from BCYE was used as the positive control and dotA - was used as the negative control.