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. 2015 Sep 25;10(9):e0139277. doi: 10.1371/journal.pone.0139277

Fig 4. Long-term survival of Lp in Fraquil and intracellular multiplication (ICM) after long-term exposure to a moderate temperature.

Fig 4

A) The JR32 strain was exposed to Fraquil and CFU counts were used to track survival over a 211-day time period. Weekly CFU counts were performed to track survival. DL, detection limit. The vertical lines indicate the time points at which the temperature of incubator was reduced from 15°C to 10°C (grey– 176 days) and when samples were harvested to infect amoeba (black– 208 days). At 4°C, CFU counts from 14 days are statistically different (P≤0.05) than CFU counts at 15°C. At 25°C, CFU counts from 113 days are statistically different than CFU counts at 15°C. At 37°C, CFU counts from 42 days are statistically different than CFU counts at 15°C. B) A. castellanii was infected with JR32 that had been exposed to 15°C for 176 days and 10°C for an additional 32 days at an MOI of 0.1. Daily CFU counts monitored the ICM inside amoeba and are presented as the ratio over CFU counts at day 0. JR32 from BCYE was used as the positive control and dotA - was used as the negative control.