Table 3. Characteristics of sequences from selected plastid regions (matK, rbcL, rpoC and trnL) and nuclear genome region (ITS1) retrieved from GenBank (NCBI) from species of Croton.
Markers | matK | rbcL1 | rbcL2 | rbcL3 | trnL | ITS1 | rpoC |
---|---|---|---|---|---|---|---|
Search result from GenBank | 62 | 148 | 148 | 148 | 240 | 770 | 0 |
Sequences included in dataset | 19 | 39 | 39 | 39 | 136 | 293 | - |
Expected product size | 125 | 100 | 149 | 145 | 101–115 | 290–320 | - |
Characters (bp) | 125 | 100 | 149 | 145 | 124 | 353 | - |
Variable characters (%) | 19 (15.2) | 14 (14) | 13 (8.72) | 12 (8.27) | 29 (23.38) | 239 (67.7) | - |
Conserved F primer sites/total (%) | 8/26 (30.77) | 20/22 (90.9) | 22/22 (100) | 24/25 (96) | 20/21 (95.24) | 23/24 (95.83) | - |
Conserved R primer sites/total (%) | 25/27 (92.59) | 24/25 (96) | 24/25 (96) | 20/21 (95.24) | 26/27 (96.29) | 20/24 (83.33) | - |
Average %GC content (SD) | 32.26 (1.02) | 53.54 (0.59) | 45.1 (0.67) | 43.56 (0.72) | 35.74 (1.1) | 52.17 (2.82) | - |