Table 2. Percentage of mutations (≥5%) emerged in the genome of A/PR/8/34 strain (CBER stock) obtained by sequencing five times its RNA library by HiSeq and MiSeq.

Notes: MiSeq values that differ from HiSeq's values are presented in parentheses. These mutation percentages were calculated per comparison to their published sequences. (See accession numbers in Materials and Methods paragraph.) nt: Nucleotide, aa: Amino-acid.

Segment	nt. Change	aa. Change	A-PR8-34 Repeat 1	A-PR8-34 Repeat 2	A-PR8-34 Repeat 3	A-PR8-34 Repeat 4	A-PR8-34 Repeat 5
PB2	279C→A	Silent (84Ala)	100	100	100	100	100
	306A→T	Silent (93Pro)	100	100	100	100	100
	1422C→A	Silent (465Pro)	100	100	100	100	100
	2127G→A	Silent (700Glu)	99	99	99	98	98
	2128A→G	701Asn→Asp	99	99	99	98	98
PB1	294G→A	Silent (90Glu)	100	100	100	100	100
	297G→A	Silent (91Ala)	100	100	100	100	100
	370C→A	116Gln→Lys				6 (<5)	5(<5)
	639G→A	205Met→Ile	100	100	100	100	100
	1002C→A	Silent (326Thr)	100	100	100	100	100
	1204T→C	394Ser→Pro	99	99	99	99	99
	1551T→C	Silent (509Leu)	99	99	99	99	99
	2076G→A	Silent (684Glu)	100	100	100	100	100
PA	323C→A	100Ala→Asp	5 (<5)	6 (<5)	5(<5)	6 (<5)	6(<5)
	323C→G	100Ala→Gly	5 (<5)	5(<5)	5(<5)	6 (<5)	6(<5)
	799C→A	259Pro→Thr	8 (5)	8 (6)	7 (5)
	873A→G	Silent (283Leu)	100	100	100	100	100
	1233T→A	Silent (403Leu)	100	100	100	100	100
	2151A→C	Silent(709Ser)	99	99	99	99	99
HA	60T→A	10Cys→Ser	100	100	100	100	100
	1042A→C	337Asn→Thr	99(100)	99(100)	99	99(100)	99(100)
NP	54T→C	Silent(3Ser)	99(100)	99(100)	99	99(100)	99(100)
	69A→G	Silent(8Arg)	100	100	100	100	100
	282T→G	Silent (79Leu)	100	100	100	100	100
	630A→G	Silent (195Arg)	100	100	100	100	100
	924G→A	Silent (293Arg)	100	100	100	100	100
	1094C→A	350Thr→Lys	22 (18)	22 (18)	21 (18)	20 (18)	22 (18)
	1350G→A	Silent(435Gly)	100	100	100	100	100
NA	43T→C	8Ile→Thr	97	97	97	96	96
	258C→A	80Arg→Ser				5(<5)	5(<5)
	314C→A	98Asp→Glu	6 (<5)	6 (<5)	6 (<5)	7(<5)	7(<5)
	412G→A	131Ser→Asn	100	100	100	100	100
	773G→A	Silent(251Ser)	100	100	100	100	100
	860T→C	Silent(280Asn)	98 (99)	98(99)	98(99)	98(99)	98(99)
	1142G→T	Silent(374Val)	100	100	100	100	100
	1211G→A	Silent (397Leu)	100	100	100	100	100
	1357C→A	446Ala→Asp			5(<5)	5(<5)	5(<5)
M	67C→A	Silent (14Ile)	9(<5)	9(<5)	9(<5)	9(<5)	9(<5)
	136C→T	Silent (37Thr)	100	100	100	99%	100
	685A→G	Silent (220Gly)	100	100	100	100	100
	697C→A	224Ser→Arg	6 <5)	6 (<5)	7(<5)	6 (<5)	6 (<5)
	792A→G	5'UTR	100	99	99	99	99
NS	105C→A	27Leu→Ile	6 (<5)	6 (<5)	6 (<5)	7(<5)	6 (<5)
	170T→C	Silent (48Ser)	100	100	100	100	100
	173T→C	Silent (49Thr)	100	100	99	100	100
	189A→G	55Lys→Glu	100	100	100	100	99(100)
	575A→G	Silent (183Gly)	100	100	100	100	100