Table 3. Percentage of mutations (≥5%) emerged in A/PR/8/34 viruses 1 and 3 genomes that were subjected to RNA library preparation followed by HiSeq sequencing.
Note: These mutation percentages were calculated per comparison of A/PR/8/34 strains 1 and 3 genomes with the corresponding published sequences. (See accession numbers in Materials and Methods paragraph.) nt: Nucleotide, aa: Amino-acid.
| Segment | nt. Change | aa. change | A/PR/8/34-1 | A/PR/8/34-3 |
|---|---|---|---|---|
| PB2 | 51A→G | Silent (8Arg) | 28 | 12 |
| 130A→C | 35Thr→Pro | 15 | ||
| 133T→G | 36Ser→Ala | 17 | 5 | |
| 139A→T | 38Arg→Stp | 6 | ||
| 142C→A | 39Gln→Lys | 6 | 5 | |
| 1083G→A | Silent (352Leu) | 5 | ||
| 1306C→A | Silent(427Arg) | 6 | ||
| 2128A→G | 701Asn→Asp | 99 | 99 | |
| 2142G→C | Silent(705Gly) | 6 | ||
| 2149C→T | Silent (708Leu) | 10 | ||
| 2199G→A | Silent (724Val) | 15 | 6 | |
| 2238G→A | Silent (737Arg) | 36 | 14 | |
| 2243G→A | 739Arg→Gln | 23 | 9 | |
| PB1 | 115A→T | 31Ser→Cys | 10 | 5 |
| 133G→A | 37Gly→Arg | 20 | 10 | |
| 179A→G | 52Lys→Arg | 18 | 9 | |
| 189G→A | 55Trp→Stp | 19 | 11 | |
| 192A→T | Silent (56Thr) | 13 | 7 | |
| 370C→A | 116Gln→Lys | 6 | ||
| 2076G→A | Silent (684Glu) | 33 | 16 | |
| 2097G→A | Silent (691Arg) | 37 | 19 | |
| 2145A→G | Silent (707Arg) | 40 | 21 | |
| 2178G→A | 718Met→Ile | 36 | 18 | |
| 2179G→T | 719Val→Phe | 35 | 16 | |
| 2236G→A | 738Glu→Lys | 46 | 23 | |
| 2267C→A | 748Ser→Tyr | 6 | 7 | |
| PA | 150G→A | Silent (42Leu) | 6 | |
| 323C→A | 100Ala→Asp | 7 | 8 | |
| 323C→G | 100Ala→Gly | 7 | 8 | |
| 873A→G | Silent (283Leu) | 99 | 99 | |
| 903G→A | Silent (293Glu) | 5 | 5 | |
| 2151A→C | Silent (709Ser) | 99 | 98 | |
| HA | 724C→A | 231Ala→Glu | 5 | |
| 1042A→T | 337Asn→Ile | 100 | 100 | |
| 1052T→C | Silent (340Ile) | 9 | 5 | |
| 1413C→A | 461Leu→Met | 5 | 5 | |
| NP | 132C→T | Silent (29Val) | 8 | |
| 184C→A | 47Leu→Ile | 6 | ||
| 1094C→A | 350Thr→Lys | 19 | 22 | |
| NA | 258C→A | 80Arg→Ser | 5 | 6 |
| 304C→A | 95Ser→Tyr | 6 | ||
| 773G→A | Silent (251Ser) | 100 | 100 | |
| 1357C→A | 446Ala→Asp | 7 | 6 | |
| M | 58A→T | Silent (11Val) | 23 | 9 |
| 67C→A | Silent (14Ile) | 6 | 9 | |
| 100A→G | Silent (25Ala) | 24 | 11 | |
| 113G→A | 30Asp→Asn | 28 | 11 | |
| 130G→A | Silent (35Lys) | 30 | 13 | |
| 240G→A | 72Arg→Gln | 26 | 10 | |
| 241A→G | Silent (72Arg) | 29 | 11 | |
| 334C→T | Silent (103Leu) | 27 | 11 | |
| 353C→T | 110His→Tyr | 25 | 10 | |
| 361C→T | Silent (112Ala) | 29 | 11 | |
| 370C→A | Silent (115Ile) | 26 | 11 | |
| 371T→G | 116Ser→Ala | 25 | 11 | |
| 385T→C | Silent (120Ser) | 22 | 9 | |
| 401A→T | 126Ser→Cys | 25 | 9 | |
| 434G→A | 137Ala→Thr | 23 | 10 | |
| 442C→T | Silent(139Thr) | 25 | 9 | |
| 443A→G | 140Thr→Ala | 28 | 10 | |
| 453C→T | 143Ala→Val | 22 | 8 | |
| 454A→G | Silent (143Ala) | 23 | 10 | |
| 524A→G | 167Thr→Ala | 26 | 12 | |
| 643T→G | Silent (206Ala) | 23 | 10 | |
| 645G→T | 207Ser→Ile | 22 | 9 | |
| 648A→G | 208Gln→Arg | 25 | 10 | |
| 655A→G | Silent (210Arg) | 27 | 11 | |
| 667A→G | Silent (214Gln) | 27 | 11 | |
| 670G→A | Silent (215Ala) | 29 | 12 | |
| 685A→G | Silent (220Gly) | 100 | 100 | |
| 697C→A | 224Ser→Arg | 7 | 8 | |
| 716A→G | 231Asn→Asp | 24 | 10 | |
| 792A→G | 5'UTR | 31 | 11 | |
| 793C→T | 5'UTR | 22 | 9 | |
| 801G→T | 5'UTR | 29 | 11 | |
| 805A→G | 5'UTR | 26 | 10 | |
| 829C→T | 5'UTR | 22 | 9 | |
| 874G→T | 5'UTR | 27 | 9 | |
| 887A→T | 5'UTR | 27 | 12 | |
| 888C→T | 5'UTR | 25 | 9 | |
| 894G→A | 5'UTR | 26 | 10 | |
| 943G→A | 5'UTR | 31 | 12 | |
| NS | 591G→A | 189Asp→Asn | 6 | 7 |
| 616C→A | 197Thr→Asn | 6 |