Table 4. HiSeq sequencing of RNA Library prepared from A/Ca/07/2009 (H1N1) virus.

Note: These mutation percentages were calculated per comparison of A/California/07/2009 (H1N1) genome with its published sequence. (See accession numbers in Materials and Methods paragraph.) aa: Amino acid, nt: Nucleotide.

Segment	nt. Change*	aa. Change	Mutation percent (≥5%)
PB2	114G→T	33Lys→Asn	7
	125G→C	37Gly→Ala	5
	129G→A	Silent (38Arg)	5
	1726C→T	Silent (571Leu)	46
PA	57A→G	18Glu→Gly	66
	121C→A	39Cys→Stp	7
HA	456A→G	146Asn→Asp	11
	736A→G	239Asp→Gly	16
	739G→A	240Arg→Gln	16
NP	335G→A	101Gly→Asp	31
	337G→A	102Gly→Arg	5
	357C→T	Silent (108Leu)	6
	374A→G	114Glu→Gly	6
	1156G→A	375Asp→Asn	15

*: Count of nucleotide location started 20 nucleotides (UTR) before the starting codon AUG.