Table 5. Mutation percentages (≥5%) emerged in HA and NA of 121XP-M4 virus passaged in eggs.

Note: These mutation percentages were calculated per comparison with the corresponding expanded wild-type progenitor. (See accession numbers in Materials and Methods.) aa: Amino-acid, nt: Nucleotide.

Segment	nt. Change	aa. Change	HiSeq		MiSeq
			DNA-Library	RNA-Library	RNA-Library
HA	47A→G	16Asn→Ser	32	36	33
	338T→C	113Ile→Thr	7	7	7
	408G→T	136Lys→Asn	79	78	78
	561G→A	Silent (187Gly)	9	10	9
	676A→G	226Lys→Glu	15	18	16
NA	166T→C	56Tyr→His	14	17	17
	248T→C	83Val→Leu	14	13	16
	255A→G	85Leu→Val	84	83	82
	262A→G	88Asn→Asp	83	81	80
	263A→G	88Asn→Asp	84	83	81
	264T→C	88Asn→His	14	17	19
	355A→G	119Lys→Glu	100	100	100
	708T→G	Silent (236Gly)	37	37	38
	1352A→G	Silent (451Asp)	35	42	38
	1354A→G	452Thr→Ala	35	47	39