Fig 2. Effect of the inhibition of Complex I of the electron transport chain on stallion sperm motility (CASA analysis).

Stallion spermatozoa were extended in BWW media and incubated in presence of rotenone (0 nm (DMSO), 100 nm, 500 nm, 5 μM and 10 μM) and antimycin-A (0 vehicle DMSO, 100 nm, 500 nm, 5 μM and 10 μM) as indicated in the materials and methods section; motility was evaluated after 1 and three hours of incubation. TM%—percentage of total motile spermatozoa, PM %—percentage of progressive motile spermatozoa. Comparisons are made against controls. * P<0.05, ** P<0.01. The results are given as the means ± SD. The graphics represent two independent experiments (one for antimycin-A and one for rotenone) following a split sample design (the same ejaculate was split into the treatment and control groups).