Fig 5. Effects of the inhibition of Complexes I and III of the electron transport chain on the percentage of stallion spermatozoa showing intact membranes.

Stallion spermatozoa were extended in BWW media and incubated in the presence of rotenone (0 nm (DMSO), 100 nm, 500 nm, 5 μM and 10 μM) or antimycin-a (0 (DMSO), 100 nm, 500 nm, 5 μM and 10 μM) as indicated in the materials and methods; membrane integrity was determined using the H33342/YoPr/Ethidium assay as described in the materials and methods. ** P<0.01. The results are given as the means ± SD.