Fig 7. Effect of the inhibition of Complexes I and III of the electron transport chain on superoxide and hydrogen peroxide production by stallion spermatozoa.
Stallion spermatozoa were extended in BWW media and incubated in the presence of rotenone or antimycin as indicated in the materials and methods. Superoxide and hydrogen peroxide were measured using flow cytometry after HE (superoxide) and H2DCFDA (hydrogen peroxide) staining as described in the materials and methods. * p<0.05, ** p<0.01. The results are given as the means ± SD.