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. 2015 Sep 25;10(9):e0138777. doi: 10.1371/journal.pone.0138777

Fig 8. Effect of the inhibition of Complexes I and III of the electron transport chain on the production of mitochondrial superoxide in stallion spermatozoa.

Fig 8

Stallion spermatozoa were extended in BWW media and incubated in the presence of rotenone or antimycin as indicated in the materials and methods. Mitochondrial superoxide was measured using flow cytometry after Mitosox staining as described in the materials and methods. Data represent Mitosox positive sperm in the whole population. * p<0.05, ** p<0.01. The results are given as the means ± SD.