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. 2015 Sep 25;10(9):e0138777. doi: 10.1371/journal.pone.0138777

Fig 9. Effects of the inhibition of Complexes I and III of the electron transport chain on the mitochondrial membrane potential of stallion spermatozoa.

Fig 9

Stallion spermatozoa were extended in BWW media and incubated in the presence of rotenone (0 (DMSO), 100 nm, 500 nm, 5 μM and 10 μM) or antimycin-A (0 (DMSO), 100 nm, 500 nm, 5 μM and 10 μM) as indicated in the materials and methods. Mitochondrial membrane potential was assessed using flow cytometry after JC-1 staining. High MMP, spermatozoa showing high mitochondrial membrane potential, Low MMP, spermatozoa showing low mitochondrial membrane potential ** P<0.01. The results are given as the means ± SD.