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. 2015 Sep 25;10(9):e0138870. doi: 10.1371/journal.pone.0138870

Fig 1. VCAM-1 is expressed in AB8/13 cells and the expression is increased upon TNFα-activation.

Fig 1

(a) RT-qPCR analysis of human VCAM-1 mRNA expression. The AB8/13 cells were differentiated for 0, 5, 10, and 15 days at 37°C and treated with TNFα (10 ng/ml) for 4 h. (b) Western blot analysis of VCAM-1 (~110-kDa) and actin (~42-kDa) (loading control) proteins expression in cell lysate of AB8/13 cells, 15 days differentiated at 37°C in the absence (-) or presence (+) of TNFα for various time points. (c) FACS analysis of VCAM-1 protein in TNFα-activated (10 ng/ml; 24 h) and quiescent AB8/13 cells incubated with mouse anti-human-VCAM-1 for 45 min at 4°C followed by incubation with fluorescently-labelled secondary antibody. *p <0.001.