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. 2015 Sep 25;10(9):e0138870. doi: 10.1371/journal.pone.0138870

Fig 2. VCAM-1 is expressed by the mouse podocyte cell line MPC-5.

Fig 2

(a) mRNA expression of VCAM-1 in the absence (-) or presence (+) of TNFα (10 ng/ml; 24 h) as analyzed by RT-qPCR analysis. (b) Western blot analysis of protein extracts of MPC-5 cells that were incubated in the absence (-) or presence (+) of TNFα (10 ng/ml; 24 h) prior to harvesting. M and control indicate respectively, pre-stained molecular weight marker and positive control (HUVEC activated with LPS for 6 h). (c) FACS analysis of VCAM-1 expression by MPC-5 cells using anti-VCAM-1 antibody by flow cytometry. *p <0.001.