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. 2015 Sep 25;10(9):e0138870. doi: 10.1371/journal.pone.0138870

Fig 4. Anti-VCAM-1-DiI-Saint-O-Somes bind to AB8/13 cells.

Fig 4

DiI-SAINT-O-Somes (a, b) and anti-VCAM-1-DiI-SAINT-O-Somes (c, d) were incubated for 4 h at 37°C with AB8/13 cells in the absence (-) (A, C) or presence (+) (b, d) of TNFα (10 ng/ml; 24 h). The nuclei were stained blue with Hoechst 33342. Original magnification 200x. (e) Quantification of binding of anti-VCAM-1-DiI-SAINT-O-Somes or DiI-SAINT-O-Somes to AB8/13 cells. Cells incubated in equal volumes of HN buffer were taken as controls and their values were subtracted from test samples. The mean fluorescence intensity (MFI) was determined by FACS and analyzed by FlowJoTM. *p <0.001.