Table 4. Summary of identified alternative spliced genes (II).

Gene ID	Life Stages								GO Annotation
	Adu a		Sch a		Cer a		Egg a
	A b	B c	A b	B c	A b	B c	A b	B c
CUFF.3457	+	+	+	+	N.A		N.A		Smad4; SMAD, mothers against DPP 4
CUFF.5359	+	-	+	-	N.A		N.A		dishevelled protein
CUFF.5727	-	+	-	+	N.A		-	+	casein kinase II beta subunit
CUFF.11194	N.A		-	+	N.A		-	+	jnk/sapk-associated protein
CUFF.11604	+	+	+	+	N.A		+	+	protein phosphatase 2C
CUFF.11720	+	+	+	+	N.A		+	+	sodium-dependent neurotransmitter transporter
CUFF.11805	+	+	+	+	N.A		N.A		erythrocyte membrane protein
CUFF.11822	+	-	+	-	N.A		N.A		phospholipase D
CUFF.11899	+	+	+	+	N.A		+	+	ubiquitin carboxyl-terminal hydrolase 12/46
CUFF.11984	+	+	+	+	N.A		N.A		Bardet-Biedl syndrome 5
CUFF.12732	+	+	+	+	-	+	+	+	survival motor neuron protein; survival of motor neuron-related-splicing factor 30
CUFF.12753	+	-	+	-	N.A		+	-	ubiquitin-protein ligase mind-bomb
CUFF.12798	-	+	-	+	N.A		-	+	hypothetical protein
CUFF.12885	+	+	+	+	-	+	+	+	*
CUFF.12947	+	-	+	-	N.A		+	-	Slc9a7 protein
CUFF.13574	+	-	+	-	N.A		N.A		hypothetical protein
CUFF.13909	+	-	+	-	N.A		N.A		cadherin
CUFF.13989	-	+	-	+	N.A		N.A		hypothetical protein
CUFF.14192	N.A		+	-	N.A		+	+	*
CUFF.14312	N.A		+	-	N.A		N.A		hypothetical protein

^a Adu: adult stage; Sch: schistosomulum stage; Cer: cercariae stage; Egg: egg stage.

^b means PCR product which contains alternative exon or intron.

^c means PCR product which doesn’t contain alternative exon or intron.

+: means correspoding band exists.

-: means correspoding band doesn’t exist.

N.A: means in the given life stage, neither of the isoforms were amplified.

*: means according to GO annotation, no specific function was annotated for the gene.