Fig 6. Effect of TBB on the deletion constructs.

(a) Uptake of 5 μM ¹⁴C-adenosine after 1 minute incubation period without (black) and with (gray) pre-incubation with TBB. Values are expressed as mean ± SE for 5 oocytes for 3 independent experiments done in triplicate. (b) Western blot of the oocytes with and without treatment with TBB probed with EGFP monoclonal antibody. (c) Confocal images of oocytes co-injected with RNA expressing WT EGFP-hENT1 and mch-TMEM after treatment with 25 μM TBB for 12 hr. (d) Confocal images of oocytes co-injected with RNA expressing WT EGFP-hENT1 and mch-KDEL. Images were taken along the plane of the microvilli (left) and within the ER (right).