Figure 1. Higher mortality but not morbidity in DSS-challenged Ccn1^dm/dm mice.

(A) Wild type and Ccn1^dm/dm mice (n=20 per genotype) were given 5% (w/v) of DSS water for 5 days followed by 14 days of recovery with normal drinking water as indicated. Survival was monitored through three cycles of DSS challenge. *P<0.05, **P<0.001 (log-rank test). The disease course of acute colitis was monitored by measuring (B) body weight change, (C) DAI, and (D) colon lengths on indicated days after initiation of DSS exposure. (E) Orally-gavaged FITC particles were detected in the serum to evaluate intestinal barrier integrity (n=3). (F) Mucosal damage was assessed by H&E staining of paraffin-embedded sections of the distal colon; histological scores are shown on right (n=4–6). Scale bar=100 μm. (G) Cell death in the distal colon was measured by TUNEL, and cells were counterstained with 4,6-diamidino-2-phenylindole (DAPI). Quantitation is shown on the right (n=3). Scale bar=50 μm. (H) MPO activity from colonic tissue (n=3–6).