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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: Mucosal Immunol. 2015 Mar 25;8(6):1285–1296. doi: 10.1038/mi.2015.19

Figure 5. WT-CCN1, but not DM-CCN1, induces IL-6 in macrophages and fibroblasts.

Figure 5

(A) IL-6 mRNA was measured by qRT-PCR and (B) IL-6 protein from conditioned media was measured by ELISA in serum-starved (overnight) I13.35 macrophages treated with 5 μg/ml of purified WT-CCN1 or DM-CCN1, or BSA for 24 hrs. (C) I13.35 macrophages were incubated with blocking mAbs against integrin αM (50 μg/ml) or β2 (50 μg/ml) 1 hr prior to treatment with WT-CCN1 for 24 hrs. IL-6 mRNA was measured by qRT-PCR. (D) IL-6 mRNA was measured by qRT-PCR and (E) IL-6 protein from conditioned media was measured by ELISA in serum-starved 18Co fibroblasts treated with 5 μg/ml of purified WT-CCN1, DM-CCN1, BSA, or 25 ng/ml of TNFα for 24 hrs. (F) 18Co fibroblasts were incubated with blocking mAb against integrin α6 for 1 hr, then treated with WT-CCN1, TNFα, or BSA for 24 hrs as above. IL-6 mRNA was measured by qRT-PCR. Data shown as mean ± SD of triplicate experiments.