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. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2015 Aug 20;35(10):2092–2103. doi: 10.1161/ATVBAHA.115.305843

Figure 6. Lipid accumulation in DCs isolated from murine atherosclerotic plaques parallels in vitro results.

Figure 6

Cells present in atherosclerotic plaques of 5 ApoE−/− mice on a high fat diet for 12 weeks were isolated and stained with monoclonal antibodies, for dissecting DC subsets, and LipidTOX-Red, for quantifying neutral lipid content. Quantitative analysis was used to delineate DC subsets. (A–E) Examples of a CD11chiMHCIIintCD11b+ moncyte-derived iDC and a CD11chiMHCIIhiCD11b+ monocyte-derived mDC (arrows, E). Cells with high CD11b expression were excluded from analysis. (F–I) Examples of CD103+CD11c+ monocyte-independent DCs (arrows, I). (J) Quantification of the LipidTOX signal per cell for each DC subset. Error bars represent the sem. * p ≤ 0.05, *** p ≤ 0.001 Kruskal-Wallis test followed by Wilcoxon rank-sum test.